Description
Phosphate buffered saline (PBS) for laboratory use
Phosphate buffered saline (PBS) is a water-based buffer containing sodium chloride and sodium phosphates. At 1X concentration and pH 7.4 it is isotonic and close to physiological pH, which makes it one of the most widely used buffers in biology and biochemistry laboratories.
In research settings it is used for washing cells and samples, diluting reagents, and as a neutral buffer in sample preparation and biochemical procedures. Supplied as a clear, colourless solution in a sealed 10ml vial for laboratory use only.
Specifications
| Name | Phosphate buffered saline (PBS) |
|---|---|
| Concentration | 1X |
| pH | 7.4 |
| Composition | Phosphate buffer with sodium chloride (NaCl, Na2HPO4, NaH2PO4) |
| Appearance | Clear, colourless liquid |
| Volume | 10ml sealed vial |
| Grade | Laboratory reagent |
Where PBS fits in the lab
Storage and handling
Store sealed in a cool, dry place away from direct light. Once opened, keep the vial tightly closed and use aseptic technique. Follow your laboratory’s validated protocol for in-use shelf life. Check compatibility with your compound and assay before use.
Frequently asked questions
What is phosphate buffered saline (PBS)?
Phosphate buffered saline is a water-based buffer containing sodium chloride and sodium phosphates. At 1X concentration and pH 7.4 it is isotonic and close to physiological pH, and it is widely used in biology and biochemistry laboratories.
What is PBS used for in the lab?
PBS is used for washing cells and samples, diluting reagents and as a neutral buffer in sample preparation and biochemical procedures.
What does 1X mean?
1X means the solution is at its working concentration and is ready to use without further dilution. Concentrated stocks, such as 10X, must be diluted before use.
What is the difference between PBS and bacteriostatic water?
PBS is a buffered salt solution held at pH 7.4. Bacteriostatic water is purified water with 0.9% benzyl alcohol as a preservative. Which one is suitable depends on your compound and protocol.
Is this PBS sterile?
This PBS is supplied as a laboratory reagent, and we make no sterility claim for it. Use aseptic technique and follow your laboratory’s protocol.
How should PBS be stored?
Store sealed in a cool, dry place away from direct light, and keep the vial tightly closed once opened.
Do you ship from the UK?
Yes. Orders placed before 3PM UK time, Monday to Friday, are normally dispatched the same day by Royal Mail Tracked 24. See our Shipping Policy.
Is this PBS for human use?
No. This product is supplied for in vitro laboratory research only. It is not for human or animal use, and we do not provide administration or medical advice of any kind.
Research use only. This product is supplied strictly for in vitro laboratory research. It is not for human or animal use, and not for diagnostic or therapeutic purposes. By purchasing, you agree to our Research Use Disclaimer.
